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rnase h1  (New England Biolabs)


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    Structured Review

    New England Biolabs rnase h1
    Rnase H1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnase+h1/RNase+H/pm41974678-191-6-8
    Average 99 stars, based on 5390 article reviews
    rnase h1 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: DICER ribonuclease removes harmful R-loops.
    Article Snippet: Article DICER ribonuclease remo ves harmful R-loops

    Control:

    Article Title: R-loop landscapes in the developing human brain are linked to neural differentiation and cell-type specific transcription
    Article Snippet: .. As a control, 15 units of RNase H1 (NEB, cat. no. #M0297) or 1 μg/μL RNase A (Life Technologies, cat. no. #AM2269) in low (0 mM) and high (500 mM) salt conditions were added to oligonucleotide pairs and incubated at 37°C for 1 hour to destroy DNA/RNA hybrids. .. All samples were purified via Phenol:Chloroform:Isoamyl alcohol (25:24:1, pH 8.0) (Invitrogen, cat. no. #15593-031) extraction and resuspended in 50 μL EB buffer (QIAGEN).

    Article Title: RNA degradation by DIS3 is a necessary step in the resolution of backtracked transcription complexes
    Article Snippet: Samples were collected at indicated timepoints following UV irradiation and washed in cold PBS. gDNA was purified using DNeasy Blood and Tissue kit (# 69504, Qiagen) according to the manufacturer’s protocol with the following changes: the optional RNase A treatment step was omitted, cell lysis was performed at 56°C for 20min shaking and the final elution was done with 50+20μl elution buffer. .. Control samples were treated with RNase H1 (#M0297, NEB) for 2h at 37°C. .. 1μg of DNA per sample is blotted onto a wet Hybond-N+ membrane (#RPN119B, Amersham) using a slot-blot apparatus (Bio-Rad).

    Incubation:

    Article Title: R-loop landscapes in the developing human brain are linked to neural differentiation and cell-type specific transcription
    Article Snippet: .. As a control, 15 units of RNase H1 (NEB, cat. no. #M0297) or 1 μg/μL RNase A (Life Technologies, cat. no. #AM2269) in low (0 mM) and high (500 mM) salt conditions were added to oligonucleotide pairs and incubated at 37°C for 1 hour to destroy DNA/RNA hybrids. .. All samples were purified via Phenol:Chloroform:Isoamyl alcohol (25:24:1, pH 8.0) (Invitrogen, cat. no. #15593-031) extraction and resuspended in 50 μL EB buffer (QIAGEN).

    Article Title: Wdr5-mediated H3K4 methylation facilitates HSPC development via maintenance of genomic stability in zebrafish.
    Article Snippet: .. RNase T1 (EN0541, Thermo Fisher Scientific, 1:200) and RNase H1 (M0297S, NEB, 1:5) were used for enzymatic treatment at 37 °C for 1 h. The 2×HBD (100 μL, 0.2 mg/mL) was incubated overnight at 4 °C. ..

    other:

    Article Title: R-loop landscapes in the developing human brain are linked to neural differentiation and cell type-specific transcription.
    Article Snippet: S9.6 cells were grown in serum-free media (ThermoFisher Scientific) and the antibody-containing supernatant was harvested by centrifuging cells at 1.5 X g for 5 minutes.

    In Vitro:

    Article Title: Bimekizumab maintenance of response through 3 years in patients with moderate-to-severe plaque psoriasis: results from the BE BRIGHT open-label extension trial.
    Article Snippet: Migrated DNA was transferred to a nylon membrane (Perkin-Elmer, NEF988001PK) in 10x SSC and probed with 32P-radiolabeled ura4 probe (TaKaRa BcaBESTTM Labeling Kit, 6046 and alpha-32P dCTP, Perkin-Elmer, BLU013Z250UC) in Ultra-Hyb buffer (Invitrogen, AM8669) at 42 C. Signal of replication intermediates was collected in phosphor-imager software (Typhoon-trio) and quantified by densitometric analysis with ImageQuantTL software (GE healthcare). .. The ‘tail signal’ was normalized to the overall signal corresponding to arrested forks (see Figure S2A for a detailed explanation) For the in vitro treatment of purified RIs with RNase H1 (NEB, M0297L), the following modifications were performed. .. Samples were treated with RNase T1 (Thermo Scientific, EN0541) instead of RNase A. Eluted DNA fractions enriched in dsDNA and ssDNA were Molecular Cell 83, 1061–1074.e1–e6, April 6, 2023 e3 both precipitated and washed with 70% EtOH.

    Purification:

    Article Title: Bimekizumab maintenance of response through 3 years in patients with moderate-to-severe plaque psoriasis: results from the BE BRIGHT open-label extension trial.
    Article Snippet: Migrated DNA was transferred to a nylon membrane (Perkin-Elmer, NEF988001PK) in 10x SSC and probed with 32P-radiolabeled ura4 probe (TaKaRa BcaBESTTM Labeling Kit, 6046 and alpha-32P dCTP, Perkin-Elmer, BLU013Z250UC) in Ultra-Hyb buffer (Invitrogen, AM8669) at 42 C. Signal of replication intermediates was collected in phosphor-imager software (Typhoon-trio) and quantified by densitometric analysis with ImageQuantTL software (GE healthcare). .. The ‘tail signal’ was normalized to the overall signal corresponding to arrested forks (see Figure S2A for a detailed explanation) For the in vitro treatment of purified RIs with RNase H1 (NEB, M0297L), the following modifications were performed. .. Samples were treated with RNase T1 (Thermo Scientific, EN0541) instead of RNase A. Eluted DNA fractions enriched in dsDNA and ssDNA were Molecular Cell 83, 1061–1074.e1–e6, April 6, 2023 e3 both precipitated and washed with 70% EtOH.



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